Enzymatic synthesis of DNA strands containing α-L-LNA (α-L-configured locked nucleic acid) thymine nucleotides
نویسندگان
چکیده
منابع مشابه
Enzymatic synthesis of DNA strands containing α-L-LNA (α-L-configured locked nucleic acid) thymine nucleotides
We describe the first enzymatic incorporation of an α-L-LNA nucleotide into an oligonucleotide. It was found that the 5'-triphosphate of α-L-LNA is a substrate for the DNA polymerases KOD, 9°N(m), Phusion and HIV RT. Three dispersed α-L-LNA thymine nucleotides can be incorporated into DNA strands by all four polymerases, but they were unable to perform consecutive incorporations of α-L-LNA nucl...
متن کاملEnzymatic incorporation of LNA nucleotides into DNA strands.
Oligonucleotides composed of naturally occurring nucleic acids are unsuitable for many therapeutic and biotechnological applications because of their poor biostability. Numerous nucleic acid analogues have therefore been synthesized for applications related to target-validation studies or therapeutic molecules. Locked nucleic acid (LNA) nucleotide monomers are nucleic acid analogues with a fixe...
متن کاملC5-Alkynyl-Functionalized α-L-LNA: Synthesis, Thermal Denaturation Experiments and Enzymatic Stability
Major efforts are currently being devoted to improving the binding affinity, target specificity, and enzymatic stability of oligonucleotides used for nucleic acid targeting applications in molecular biology, biotechnology, and medicinal chemistry. One of the most popular strategies toward this end has been to introduce additional modifications to the sugar ring of affinity-inducing conformation...
متن کاملComputational Investigation of Locked Nucleic Acid (LNA) Nucleotides in the Active Sites of DNA Polymerases by Molecular Docking Simulations
Aptamers constitute a potential class of therapeutic molecules typically selected from a large pool of oligonucleotides against a specific target. With a scope of developing unique shorter aptamers with very high biostability and affinity, locked nucleic acid (LNA) nucleotides have been investigated as a substrate for various polymerases. Various reports showed that some thermophilic B-family D...
متن کاملThermal stability of G-rich anti-parallel DNA triplexes upon insertion of LNA and α-L-LNA.
G-rich anti-parallel DNA triplexes were modified with LNA or α-L-LNA in their Watson-Crick and TFO strands. The triplexes were formed by targeting a pyrimidine strand to a putative hairpin formed by Hoogsteen base pairing in order to use the UV melting method to evaluate the stability of the triplexes. Their thermal stability was reduced when the TFO strand was modified with LNA or α-L-LNA. The...
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ژورنال
عنوان ژورنال: Artificial DNA: PNA & XNA
سال: 2012
ISSN: 1949-095X,1949-0968
DOI: 10.4161/adna.19272